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Xiuning HUA,Wei WANG,Feng WANG
《环境科学与工程前沿(英文)》 2015年 第9卷 第6期 页码 1130-1138 doi: 10.1007/s11783-015-0821-y
关键词: chemical looping combustion iron-based oxygen carriers reduction kinetics carbon monoxide statistics
Xinghong Duo, Lingchuang Bai, Jun Wang, Jintang Guo, Xiangkui Ren, Shihai Xia, Wencheng Zhang, Abraham Domb, Yakai Feng
《化学科学与工程前沿(英文)》 2020年 第14卷 第5期 页码 889-901 doi: 10.1007/s11705-020-1919-8
关键词: gene carriers multitargeting function ECs transfection efficiency
夏家辉
《中国工程科学》 2000年 第2卷 第11期 页码 1-11
介绍了中国医学遗传学国家重点实验室在遗传病家系收集、疾病基因定位、疾病基因克隆和疾病基因功能研究方面的研究工作。用细胞遗传学G显带技术于1975年发现了一条与鼻咽癌相关的标记染色体t(1;3)(q44;p11);1981年将睾丸决定基因(TDF)定位于Yp11.32带;1991年以来收集遗传病家系345种共590个;1996年用显微切割、PCR、微克隆技术克隆了EXT2基因;1998年用基因家族-候选疾病基因克隆方法克隆了遗传性神经性耳聋基因GJB3;1999年用连锁分析和全基因组扫描将一种遗传性弥漫性浅表性光敏性汗孔角化症定位于12q23.2带,并在基因功能研究中发现了一个新的细胞内转运蛋白。
关键词: 遗传病家系 基因定位和克隆 基因家族-候选疾病基因克隆 基因组扫描 基因功能研究
Molecular engineering of dendrimer nanovectors for siRNA delivery and gene silencing
Yu Cao, Xiaoxuan Liu, Ling Peng
《化学科学与工程前沿(英文)》 2017年 第11卷 第4期 页码 663-675 doi: 10.1007/s11705-017-1623-5
关键词: gene therapy RNAi therapeutics dendrimer nanovectors gene silencing
《环境科学与工程前沿(英文)》 2021年 第15卷 第3期 doi: 10.1007/s11783-020-1335-9
•ZnO/Perlite inactivated 72% of bioaerosols in continuous gas phase.
关键词: Immobilized catalysts Continuous flow Photocatalysis Bioaerosols Cytotoxicity Inactivation mechanism
The antibiotic resistome: gene flow in environments, animals and human beings
null
《医学前沿(英文)》 2017年 第11卷 第2期 页码 161-168 doi: 10.1007/s11684-017-0531-x
The antibiotic resistance is natural in bacteria and predates the human use of antibiotics. Numerous antibiotic resistance genes (ARGs) have been discovered to confer resistance to a wide range of antibiotics. The ARGs in natural environments are highly integrated and tightly regulated in specific bacterial metabolic networks. However, the antibiotic selection pressure conferred by the use of antibiotics in both human medicine and agriculture practice leads to a significant increase of antibiotic resistance and a steady accumulation of ARGs in bacteria. In this review, we summarized, with an emphasis on an ecological point of view, the important research progress regarding the collective ARGs (antibiotic resistome) in bacterial communities of natural environments, human and animals, i.e., in the one health settings. We propose that the resistance gene flow in nature is “from the natural environments” and “to the natural environments”; human and animals, as intermediate recipients and disseminators, contribute greatly to such a resistance gene “circulation.”
Reflections on the system of evaluation of gene-edited livestock
Ziyao FAN, Tianwen WU, Kui WU, Yulian MU, Kui LI
《农业科学与工程前沿(英文)》 2020年 第7卷 第2期 页码 211-217 doi: 10.15302/J-FASE-2019303
The rapid development of biotechnology has provided a greater understanding of the biological functions of major candidate genes that have important functions regarding economic traits, and new materials for livestock breeding have been obtained through gene editing (GE) and embryo manipulation with the purpose of improving quality and output and reducing the costs and risk of disease. Public concerns, particularly over safety risks and production performance, must be addressed. Evaluation is the most important component of the regulation of gene-edited livestock and is a crucial guarantee of public safety before the marketing of gene-edited animal products. Here, the system of evaluation of gene-edited livestock is discussed in terms of public safety and production performance. The search for safe and ethical applications in the GE of livestock, a case-by-case evaluation strategy, and classification and simplification are used in order to promote a more efficient, objective, comprehensive and operable evaluation system.
《医学前沿(英文)》 2022年 第16卷 第4期 页码 627-636 doi: 10.1007/s11684-020-0815-4
关键词: RUNX1 gene mutation acute myeloid leukemia transcriptional repression DNA methylation
Construction of lentiviral vector carrying Rab9 gene and its expression in mouse brain
Youguo HAO, Min ZHANG, Jinzhi XU, Bitao BU, Jiajun WEI
《医学前沿(英文)》 2009年 第3卷 第2期 页码 141-147 doi: 10.1007/s11684-009-0041-6
Jie ZHU,Wei WANG,Xiuning HUA,Zhou XIA,Zhou DENG
《环境科学与工程前沿(英文)》 2015年 第9卷 第6期 页码 1117-1129 doi: 10.1007/s11783-015-0812-z
关键词: CO2 capture chemical looping hydrogen generation iron based oxygen carriers single packed bed reactor long-term test complex gases fuel
Nan Zheng, Ziyuan Song, Yang Liu, Lichen Yin, Jianjun Cheng
《化学科学与工程前沿(英文)》 2017年 第11卷 第4期 页码 521-528 doi: 10.1007/s11705-017-1612-8
关键词: α-helical polypeptide plant gene delivery protoplast intact leaves transfection
Profiling influences of gene overexpression on heterologous resveratrol production in
Duo Liu,Bingzhi Li,Hong Liu,Xuejiao Guo,Yingjin Yuan
《化学科学与工程前沿(英文)》 2017年 第11卷 第1期 页码 117-125 doi: 10.1007/s11705-016-1601-3
关键词: resveratrol aromatic amino acid DNA assembly metabolic engineering gene overexpression
Stem cell gene therapy: the risks of insertional mutagenesis and approaches to minimize genotoxicity
Chuanfeng Wu, Cynthia E. Dunbar
《医学前沿(英文)》 2011年 第5卷 第4期 页码 356-371 doi: 10.1007/s11684-011-0159-1
关键词: gene therapy hematopoietic stem cells insertional mutagenesis genotoxicity induced pluripotent stem cell
Optimized human factor IX expression cassettes for hepatic-directed gene therapy of hemophilia B
null
《医学前沿(英文)》 2015年 第9卷 第1期 页码 90-99 doi: 10.1007/s11684-015-0390-2
Gene therapy provides a potential cure for hemophilia B, and significant progress has been achieved in liver-directed gene transfer mediated by adeno-associated viral vectors. Recent clinical trials involving the use of a self-complementary adeno-associated virus serotype 8-human codon-optimized factor IX (AAV8-hFIXco) vector demonstrated encouraging efficacy with hFIX expression stabilized at 1% to 6% of normal level in patients, but safety concerns related to high vector doses are still present. Thus, further improvement of AAV vectors and hFIX expression cassette may positively contribute to the ultimate success of hemophilia B gene therapy. In this study, to obtain a higher expression level of hFIX that potentiates the coagulant capacity of recipients, human FIX expression vector was optimized by upgrading the codon adaption index and adjusting the GC content, inserting a Kozak sequence (GCCACC), and introducing a gain-of-function mutation, R338L (FIX Padua). The efficiency of the published and the presently constructed cassettes was compared through in vivo screening. In addition, the regulatory elements that control the FIX gene expression in these cassettes were screened for liver-specific effectiveness. Among all the constructed cassettes, scAAV-Pre-hFIXco-SIH-R338L, which was the construct under the control of the prothrombin enhancer and prealbumin promoter, resulted in the highest level of coagulant activity, and the expression levels of two constructed cassettes (scAAV-Chi-hFIXco-SIH-R338L and scAAV-Pre-hFIXco-SIH-R338L) were also higher than that of the published cassette (scAAV-LP1-hFIXco-SJ). In summary, our strategies led to a substantial increase in hFIX expression at the protein level or a remarkably elevated coagulant activity. Thus, these reconstructs of hFIX with AAV vector may potentially contribute to the creation of an efficacious gene therapy of hemophilia B.
关键词: factor IX hemophilia B liver-specific regulatory elements hydrodynamic gene transfer
null
《医学前沿(英文)》 2013年 第7卷 第3期 页码 280-289 doi: 10.1007/s11684-013-0265-3
Many gene fusions have been recognized as important diagnostic and/or prognostic markers in human malignancies. In recent years, novel gene fusions have been identified in cases without prior knowledge of the genetic background. Accompanied by a powerful computational data analysis method, new genome-wide screening approaches were used to detect cryptic genomic aberrations. This review focused on advanced genome-wide screening approaches in fusion gene identification, such as microarray-based approaches, next-generation sequencing, and NanoString nCounter gene expression system. The fundamental rationale and strategy for fusion gene identification using each biotech platform are also discussed.
关键词: gene fusion cancer microarray next-generation sequencing NanoString nCounter system
标题 作者 时间 类型 操作
Performance and kinetics of iron-based oxygen carriers reduced by carbon monoxide for chemical looping
Xiuning HUA,Wei WANG,Feng WANG
期刊论文
Multifunctional peptide conjugated amphiphilic cationic copolymer for enhancing ECs targeting, penetrating and nuclear accumulation
Xinghong Duo, Lingchuang Bai, Jun Wang, Jintang Guo, Xiangkui Ren, Shihai Xia, Wencheng Zhang, Abraham Domb, Yakai Feng
期刊论文
Molecular engineering of dendrimer nanovectors for siRNA delivery and gene silencing
Yu Cao, Xiaoxuan Liu, Ling Peng
期刊论文
bioaerosol cells to photocatalytic inactivation with ZnO and TiO impregnated onto Perlite and Poraver carriers
期刊论文
Reflections on the system of evaluation of gene-edited livestock
Ziyao FAN, Tianwen WU, Kui WU, Yulian MU, Kui LI
期刊论文
Distinct gene expression pattern of mutations coordinated by target repression and promoter hypermethylation
期刊论文
Construction of lentiviral vector carrying Rab9 gene and its expression in mouse brain
Youguo HAO, Min ZHANG, Jinzhi XU, Bitao BU, Jiajun WEI
期刊论文
Profiling influences of gene overexpression on heterologous resveratrol production in
Duo Liu,Bingzhi Li,Hong Liu,Xuejiao Guo,Yingjin Yuan
期刊论文
Stem cell gene therapy: the risks of insertional mutagenesis and approaches to minimize genotoxicity
Chuanfeng Wu, Cynthia E. Dunbar
期刊论文
Optimized human factor IX expression cassettes for hepatic-directed gene therapy of hemophilia B
null
期刊论文